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| Section | Objectives |
|---|---|
| Clinical Microbiology | - Parasitology
|
| Infectious Disease Principles | - Epidemiology
|
| Laboratory Management & Quality Systems | - Regulatory compliance
|
| Laboratory Operations | - Instrumentation and methodologies
|
1. Which pattern on a Kirby-Bauer disk diffusion plate, showing resistance of Staphylococcus aureus to erythromycin but susceptibility to clindamycin with a flattening of the clindamycin zone adjacent to the erythromycin disk (D-zone test positive), indicates inducible clindamycin resistance?
A) Indicates the strain possesses an erm gene and should be reported as clindamycin resistant
B) Indicates the strain should be reported as clindamycin susceptible
C) Indicates the strain possesses the msrA gene
D) Indicates the strain is tolerant to clindamycin
2. A research team is investigating the host immune response to Mycobacterium tuberculosis. They discover that a specific mycobacterial glycolipid inhibits the maturation of phagosomes containing the bacteria, preventing their fusion with lysosomes. This mechanism allows M. tuberculosis to:
A) Escape from the phagolysosome into the cytoplasm.
B) Survive and replicate within macrophages.
C) Evade detection by Toll-like receptors on macrophages.
D) Induce the production of pro-inflammatory cytokines.
3. A patient with a history of chronic sinusitis develops a severe invasive fungal infection involving the nasal sinuses and extending into the orbit. Tissue biopsy reveals broad, aseptate hyphae with irregular branching.
The patient is diabetic and acidotic. The MOST likely causative organism belongs to the genus:
A) Aspergillus
B) Scedosporium
C) Mucor or Rhizopus
D) Fusarium
4. A microbiology laboratory receives a corneal scraping from a patient with keratitis. Microscopic examination with calcofluor white stain reveals the presence of septate hyphae and conidiophores with a foot cell and phialides. The MOST likely genus is:
A) Candida
B) Aspergillus
C) Rhizopus
D) Mucor
5. A clinical microbiology laboratory is performing Gram staining on a sputum sample. After adding the crystal violet stain, a delay in the washing step with decolorizer (alcohol-acetone) would MOST likely result in:
A) Poor adherence of the stain to Gram-positive bacteria.
B) Increased background staining.
C) Gram-positive bacteria appearing pink.
D) Gram-negative bacteria appearing purple.
Solutions:
| Question # 1 Answer: A | Question # 2 Answer: B | Question # 3 Answer: C | Question # 4 Answer: B | Question # 5 Answer: D |
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